The Carcinogenic Activity of 2-Acetaminofluorene III. Manner of Administration, Age of Animals, and Type of Diet

Wilson, DeEds, and Cox (4) have shown that 2-acetaminofluorene (AAF), when incorporated into the diet and fed to rats for some time, produced hyperplastic and carcinomatous lesions in the animals. At the time that report was written, certain animals that had received the compound by various other routes of administration were still alive and well. The authors drew the tentative conclusion that "the failure to produce tumors in the parenterally treated rats [suggests] that continued administration by way of the gastrointestinal tract is necessary for carcinogenic activity." The preliminary work on this phase of the subject was continued and expanded, and the results form the subject matter for this paper.